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Although the growth rates of our two model organisms, at least in monoculture (approximately 26 and 31 min for P. aeruginosa and E. coli, resp ., were similar, a higher attachment for P. aeruginosa was found.
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It is because of the faster blocking of the pores in the surface layers (low interconnectivity) that they can provide a higher attachment site for cells and thus increase the viability.
For 0.5 µm, rod shaped particles exhibited significantly higher attachment compared to spheres and oblate ellipsoids.
For 3 µm particles, an opposite trend was observed where spheres showed higher attachment compared to rods and oblate ellipsoids.
For 1 µm particles, oblate ellipsoids showed highest attachment tendency followed closely by rods and then by spheres.
For all sizes and shapes studied, particles possessing the longest dimension in the range of 2 3 µm exhibited highest attachment.
Here we report the development of a high-resolution microscope attachment for camera-enabled mobile phones that is capable of both brightfield and fluorescence imaging.
Apparently, the sensitive cell lines have higher tendency for attachment, as the adhesion related genes are upregulated in these, whereas the resistant cell lines are significantly more responsive to endogenous and exogenous stimuli.
Soaking could have been one potential explanation for the higher bacterial attachment, but it is not the case with these substrates.
We have developed a microscope attachment for a camera-enabled mobile phone such that it can be used as a platform for high-resolution clinical light microscopy.
"I feel strong attachment for the armed forces.
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