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Again, DFAs using pooled acoustic parameters of both call parts showed higher assignment probabilities than DFAs on one part only.
DFAs using pooled acoustic parameters of both call parts showed higher assignment probabilities (71.3% correct assignment rate) than DFAs that only included call parameters of part 1 (48.8%) or part 2 (45.0%),.
If genes could be assigned to more than one group we applied a priority-ruling scheme: 5PP > EE > 5PI-EI > COS > CSS > SG; where '>' is a placeholder for 'has higher assignment priority than'.
Majority of samples (WP 3-WP 6) were not well-resolved and assigned to both K 1.1 and K 1.2, however samples WP 1 and WP 7 had higher assignment probability to K 1.1 (0.80 for WP1, and 0.825 for WP7).
Although DFAs using differing numbers of variables are not directly comparable, the higher assignment rate of the DFAs using acoustic parameters of both call parts demonstrates that a composite call, i.e. increased complexity within a signal, provides enhanced discrimination compared to each call part alone.
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We initially fit a Gaussian mixture model to the contrast dimension data to find the center of each genotype group and to assign genotypes to those samples having high assignment probability.
However, the high assignment accuracy (correct hits, %TP (Supplementary Information Table S1)) means that practically all assigned reads were correctly classified by MEGAN.
The experimental results using all the six microphone arrays deployed in the room show a high assignment rate in our particular scenario.
According to a study by Menna Jones, "gene flow appears extensive up to 50 km", meaning a high assignment rate to source or close neighbour populations "in agreement with movement data.
Likewise, existence of samples with a very high assignment percentage probability does not rule out an interspecific origin.
In the current study, we identified six females and three males of mixed ancestry that exhibited high assignment probabilities to the extinct Floreana species.
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