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When a high-resolution typing approach based on loci with fast mutation rates, such as MLVA, is used, near matches should be the rule rather than the exception.
In spite of many available complete genomes, this wealth of information has not been translated into a WGS-informed high-resolution typing scheme.
All had high-resolution typing for HLA-A, -B, -C, and -DRB1.
High-resolution typing, such as that required during outbreak investigations, usually requires the inclusion of genes with greater diversity, e.g. antigen genes, rather than housekeeping genes [15].
High-resolution typing of HLA-A,-B,-C in DD8 cells was performed and confirmed by cloning and sequencing the relevant exons within the HLA loci.
SBT provides high-resolution typing, but at high cost and low throughput, and is not able to discern cis/trans linkage of sequence motifs, which can result in ambiguities in allele calls [43].
Similar(28)
Virtual donor searches for 1492 high-resolution typed Finnish patients in the Finnish, Swedish and German registries were then performed, using individual match predictions for each registry.
For further resolution of the patient's DR and DQ typings we performed NGS high resolution typing using Illumina TruSight v2 sequencing panel.
Both methods are very useful for clinical and research purposes and can provide generic (low resolution) to allelic (high resolution) typing results [24].
Whole-genome sequencing (WGS) is the highest-resolution typing method available for transmission epidemiology.
Lastly, the smallest set of ultraspecific probes is selected such that the highest resolution typing can be achieved with a minimal cost in producing the assay.
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