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Chen, S. et al. High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy.
The goal is to generate high-resolution noise source maps with self-consistency, i.e., their integration over the extent of the noise source region gives the far-field pressure auto-spectrum for a particular emission direction.
FSC curves were calculated from data half-sets using soft masks and corrected for the effect of the mask using high-resolution noise substitution.
All reported resolutions are based on the gold-standard FSC = 0.143 criteria (Scheres and Chen, 2012), and the final FSC curve were corrected for the effect of a soft mask using high-resolution noise substitution (Chen et al., 2013).
High-resolution noise will be uncorrelated between the two maps.
Fourier Shell Coefficient (FSC) curves were corrected for the effects of a soft mask on the FSC curve using high-resolution noise substitution (Chen et al., 2013).
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All final maps were assessed for possible over-fitting during refinement using a high-resolution noise-substitution test (Chen et al., 2013).
Reported resolutions are based on the gold-standard FSC-0.143 FSC-0.143 (Scriteriond Chen, 2012) and FSchereses were corrected for the convolution effects of andoft mask using high-resolution noise-substitution (Chen et al., 2012).
To ensure the low non-linearity and high resolution, noise analysis and non-linearity simulation are conducted.
The SS-MT method produces high-resolution, low-noise spectrograms from such data.
A high-resolution low-noise single-laser photonic filter that can operate to high frequencies is presented.
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