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The application of a MiSeq sequencing platform, which is a bench-top high-throughput sequencer, allowed >1.3 Gb of high-quality sequencing data to be produced at a low cost in a short time.
A key innovation is the design of a new forward primer for the specific PCR amplification and high-quality sequencing of cyt b.
High-quality sequencing approaches have been suggested in conjunction with high-throughput sequencing for comparative genomics analyses and genome evolution studies (Alkan et al. [2011]).
The main challenges at this point are to develop robust, high-throughput pipelines to generate high-quality sequencing libraries from single cells, and to establish a thorough understanding of the potential pitfalls of the approach.
This finding demonstrates a high-quality sequencing dataset.
Based on the high-quality sequencing we could assemble the S. carlsbergensis genome into just 78 scaffolds.
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High-quality sequences were used as input.
These numerical values represent that high-quality sequences were obtained for further analysis.
High-quality sequences were used for downstream analysis, and chimera sequences were removed with the UCHIME algorithm.
Celera's idea was to make money by selling access to its high-quality sequence database.
Following ITU P.910 recommendations [7], it is the comfortable distance according to high-quality sequences.
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