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Both MetaVelvet and IDBA-UD have been shown to produce longer high-quality assemblies than single-genome assemblers, such as Velvet and SOAPdenovo2.
A good assembler should generate high-quality assemblies that contain as many reference transcripts as possible, and each reference transcript should be covered as completely as possible with a single long contig rather than a combination of several short contigs.
Benchmarks across eight popular assemblers demonstrate that exSPA nder produces high-quality assemblies for datasets of different types.
Genome assembly was performed using ALLPATHS-LG (Broad Institute of MIT and Harvard), a graph-based assembler that has been shown to produce high-quality assemblies for medium- to large-sized genomes (Gnerre et al. 2011).
Therefore, even high-quality assemblies would typically sequence to no greater than 10x depth of coverage.
Though the de novo sequencing of bacterial genomes using Pyrosequencing and a whole-genome shotgun approach has been demonstrated [28], producing high-quality assemblies continues to be a challenge for bacterial genomes [29], and it seems unlikely that such an approach would extend to complex eukaryotes.
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This approach depends on having a high-quality assembly, as it is not possible to find large structural variations if the assembly is highly fragmented.
American corporations, for example, are "offshoring" whole stages of production to East Asia, particularly the Chinese coast, where they can find cheap, high-quality assembly lines and back offices.One cause behind this transfer of jobs is East Asian currency policy.
The high-quality assembly and annotation of the O. glaberrima genome have also been reported, providing evidence for its independent domestication (Wang et al. 2014).
Inspection of this scaffold showed that the sequence corresponding to the anole protocadherin cluster represents a high-quality assembly region interrupted by 24 gaps.
Overall, these comparisons also suggest a high-quality assembly.
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