High-quality nucleic acid extraction is necessary for the multiplex RT-PCR assays as the results are greatly influenced by the nucleic acid quality [ 12, 13].
Novozymes has been able to retrain its bacillus bacteria to produce high-quality hyaluronic acid without using animals or pathogens.
One (henceforth known as Subject A) was on a hit of high-quality blotter acid.
High-quality hydrochloric acid is used in the regeneration of ion exchange resins.
OH− + HCl → H2O + Cl− In industry demanding purity (food, pharmaceutical, drinking water), high-quality hydrochloric acid is used to control the pH of process water streams.
The presence of endonucleases within human neutrophils, a part of the host defense against bacteria [ 8], presents particular challenges to preparing high-quality nucleic acid for sequencing studies.
While reverse-transcriptase polymerase chain reaction (RT-PCR) is highly specific and capable of providing details about exonic structure, it only detects fusion breakpoints for which the assay is designed, and requires high-quality ribonucleic acid (RNA) that is often not available from formalin-fixed paraffin-embedded (FFPE) specimens.
Successful molecular-based studies rely on extraction of high-quality nucleic acids from complex samples (i.e. production waters, biofilm material, sediment, etc).
Commercially available kits yield high-quality nucleic acids, but time-consuming sample processing and the requirements for additional equipment largely limits their use to molecular biology labs.
Isolating high-quality nucleic acids from blood is part of many diagnostic, forensic and scientific applications ranging from: pathogen detection in livestock and humans such as HIV, HBV, HCV and bacteria [ 1– 5]; forensics identification [ 6]; disease diagnosis (such as cancer) [ 7– 10]; genetic disorders [ 6, 11, 12]; and neonatal methylation studies [ 13].
However, different microalgae strains show different efficiencies in terms of lipid extraction where the Soxhlet method is normally used for high-quality lipids (fatty acid and triglycerides), whereas the Bligh Dryer method is normally used for extracting total lipids from microalgae.
