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These stiffer hydrogels have rheological properties more suited to bioprinting high-density cell suspensions.
In vivo nuclear magnetic resonance (NMR) monitoring requires a high-density cell suspension, where cell precipitation should be avoided.
High-density cell suspensions, which had an OD600 of 30 and a total volume of 80 ml, were cultured at 35 and 40°C under anaerobic conditions.
Feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply of the NAD+ and NADPH cofactors.
For manufacturing on an industrial scale, it is preferable to use unconcentrated culture broth as the source of enzymes, and therefore, a high-density cell culture is required.
Then, solvent was directly added to 5%v/v, exposed to a high-density cell for 6 h and cell viability was determined as colony-forming unit per milliliter (CFU ml-1).
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The present results will be useful for system designers of modern broadband wireless access (BWA) and high-density cell-based Ku/Ka, Q/V band satellite systems, over the desired area of coverage in order to determine the appropriate effective isotropically radiated power (EIRP) and receiver characteristics of this region.
High-density cell-based arrays were successfully fabricated using 1,959 un-tagged open reading frames (ORFs) taken from the Mammalian Gene Collection (MGC) in mammalian expression vectors.
MSCs were suspended in the collagen solution at room temperature to a final cell density of 4 × 10/ml and 100 μl gels formed in 0.4-μm high-density cell-culture inserts (BD Biosciences, San Jose, CA, USA).
High-density cells were greater than 7×10 cells/ml.
As reported previously, low-density cells showed significant abscission delays as compared to high-density cells.
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