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To guarantee high viability, cells were prepared as previously detailed [23].
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Seger et al. demonstrated such a technology for semi-automated, high-viability cell injection [77].
NIH/3T3 normal cells and B16F10 melanoma cell lines were treated with PDT and presented 85% cell death for melanoma cells, while maintaining high viability in normal cells.
In the absence of DOX, all strains showed high viability and rapid cell cycle progression when plated to YPD as single G1 cells (Fig. 1D).
At 12 h of exposure, the highest viability of cells was recorded: 6 ± 10.03 × 104/ml, which was consistently the same in all concentrations of exposure.
However, treatment with 10 µM hippuristanol confers significantly higher viability upon cells undergoing oxidative stress compared with those in which eIF4A is uninhibited (figure 5).
The reason for the higher viability of cells after freezing relative to post-freeze drying is probably associated with the non-optimal conditions of the drying or storage process.
In vitro cell assays demonstrated that while preosteoblasts had high cell viability and cell spreading on the fibrous nanohybrids, cell metabolic activity can also increase with increasing incubation time.
We demonstrate that these new biohybrid materials are naturally remodeled by mesenchymal stem cells, while supporting high viabilities and affecting cell morphology and organization.
The cells maintained high viability and showed distinct morphology.
(2) They form spores that can be easily formulated, and have high viability in comparison with vegetative cells.
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