Optimizing the selection phase for transformed A. fimbriata explants would facilitate a high throughput transformation system for investigating gene function and evolution in a basal angiosperm.
PX, XG, LC, XW, YD, JX, JP, VS, MT and KE constructed mutants; PX designed primers and performed bioinformatics analysis; XG established the high throughput transformation system and performed microarray and gene functional analysis; XW performed double-knockout and mutant growth comparison; PX, XG, LC, JX, TK and DB analyzed the data.
This investigation sought to improve still further transformation efficiencies and to provide a reliable high efficiency transformation system for L. lactis spp. lactis.
In an attempt to develop a simple, high efficiency transformation system mediated by A. tumefaciens in M. thermophila ATCC 42464, we performed transformation optimization as described previously [ 21].
Therefore, a high frequency transformation system is essential, with a ready supply of selectable marker genes to allow multiple constructs to be expressed, and the ability to carry out targeted mutations (Kilaru and Steinberg, in 2015; Kilaru et al., 2015a; Schuster et al., 2015a,b; Mehrabi et al., 2015).
For such species, higher throughput transformation systems are needed to be able to fully benefit from the rapid development of plant genomics for basic research and for the design of new genetic improvement strategies including both conventional breeding and genetic transformation.
The development of a reliable, high-efficiency genetic transformation system for D. salina is an important objective for the Dunaliella research community, especially when considering the many industrial applications that this technology could provide.
In the present study, we developed a high efficiency Agrobacterium tumefaciens mediated transformation system for M. thermophila, including an approach for targeted gene deletion using green fluorescence protein (GFP) as a marker for selection.
Moreover, in comparison with other major cereals like rice and maize, the development of a high throughput wheat transformation systems has been slowed and severely affected by genotype effects on plant regeneration, low transformation efficiencies and problems with transgene inheritance and stability of expression.
These promoter constructs were analyzed using an Arabidopsis system since it has high transformation efficiency and there is a large amount of similarity between these two species.
