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Therefore, we attempted to elucidate the mechanism of high transformation frequency in CAG18439 as follows (Table 7 and Figs. 3 6).
These results suggest that unidentified mutation(s) in the CAG18439 chromosome other than Tn10 and lacI cause the high transformation frequency in CAG18439.
In high-frequency combinations, it was observed that CAG18439 caused high transformation frequency not only as the recipient cell but also as the donor cell (Tables 2 and 3).
If deletion of an ACS resulted in loss of the high transformation frequency of the ARS, the ACS was denoted as functional.
In the present investigation, we have been able to successfully introduce marker genes into bread wheat and durum wheat with a high transformation frequency.
These growth characteristics and the high transformation frequency of A. rhizogenes have made the production of 'composite plants' in vitro and ex vitro a tool to test gene functions for root biology [ 8- 10].
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High transformation frequencies were observed for all the cassettes, generating usually 10 15 independent transplastomic clones after bombardment of five sterile-grown tobacco leaves.
The hypervirulent A. tumefaciens strain AGL1 carrying the binary vector pDM805 containing the bar gene under the control of the Ubi1 promoter and the uidA gene driven by Act1 promoter was used for transforming four different explant types of which somatic embryos gave the highest transformation frequency [ 47].
As shown in Figure 7A, the highest transformation frequency (91.54%) was achieved with a 2-day co-cultivation.
The highest transformation frequency (74.64%) was obtained when the stem sections with one node were used as explants.
The highest transformation frequency was found when the co-cultivation was carried out at 22°C in this study.
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