Your English writing platform
Discover LudwigSuggestions(1)
Exact(28)
In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.
Both genes were successfully transformed into Y. lipolytica Po1g with a high transformation efficiency, about 3,000/μg DNA.> -wrap-foot> The strain used for the enzyme expression was Y. lipolytica Po1g (MATa ,leu2-270 ,leu2-270 ,ura3-302 ,ura3-302 URA3 ,xpr2-332 ,xpr2-33227].
In addition, yeasts are attractive hosts for the high throughput screening of peptides or proteins from large combinatorial libraries (Chao et al. 2006; Li et al. 2007; Ueda 2004), with a high transformation efficiency of plasmids of up to 1 1.5 × 108 transformants/μg achieved (Benatuil et al. 2010).
A maximum number of transformants with a minimal amount of DNA requires a high transformation efficiency.
High transformation efficiency and accuracy were achieved as ~63% of the transformants was confirmed to be correct.
These numbers, coupled with the need for efficient selection procedures, limit applicability to organisms possessing high transformation efficiency and favorable growth characteristics.
Similar(32)
If not the best producer, Yarrowia offers very high transformation efficiencies, allowing the genetic engineering of laccases for industrial applications.
Vogel and Hill [ 22] identified an inbred diploid line, Bd21-3, withighgh transformation efficiencies.
Also, their thick cell walls preclude the high transformation efficiencies necessary to screen complex SynE libraries.
The SLiCEs from E. coli laboratory strains in this study have such high transformation efficiencies because several steps of the original SLiCE preparation protocol [ 14] have been modified.
Although the spheroblast method results in high transformation efficiencies, it is too much time-consuming for large-scale experiments like library screenings.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com