In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.
Both genes were successfully transformed into Y. lipolytica Po1g with a high transformation efficiency, about 3,000/μg DNA.> -wrap-foot> The strain used for the enzyme expression was Y. lipolytica Po1g (MATa ,leu2-270 ,leu2-270 ,ura3-302 ,ura3-302 URA3 ,xpr2-332 ,xpr2-33227].
In addition, yeasts are attractive hosts for the high throughput screening of peptides or proteins from large combinatorial libraries (Chao et al. 2006; Li et al. 2007; Ueda 2004), with a high transformation efficiency of plasmids of up to 1 1.5 × 108 transformants/μg achieved (Benatuil et al. 2010).
A maximum number of transformants with a minimal amount of DNA requires a high transformation efficiency.
High transformation efficiency and accuracy were achieved as ~63% of the transformants was confirmed to be correct.
Gene disruption and replacement in Corynebacterium glutamicum is dependent upon a high transformation efficiency.
If not the best producer, Yarrowia offers very high transformation efficiencies, allowing the genetic engineering of laccases for industrial applications.
BF variants with small misorientations are formed side by side at high transformation temperatures by sharing the same Bain correspondence.
Thermo-mechanical analysis (TMA) results demonstrated relatively high transformation temperature of the OC, which explains well its superior anti-sintering property.
We also found high transformation potential reflecting functional redundancy among communities within each meta-community, and across meta-communities in the landscape.
In contrast, in aprotic solutions, a softened binder easily accommodates the high transformation strain, ensuring excellent electrode cycling performance (soft and tough behavior).
