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Size control of MCTS is a major challenge in obtaining uniform and reliable high throughput test systems; various techniques such as forced aggregation techniques, micro textured surfaces, and porous 3D scaffolds are being employed to solve these issues [ 163– 163].
We suggest that our approach of studying the response to 5FU in a large panel of well-characterised cell lines, using a relatively high throughput test procedure and an objective categorisation of response, could be a model for preclinical testing of other drugs.
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Moreover, accurate high throughput testing may help tumor classification in terms of prognosis and drug sensitivity.
This is unfeasible for high throughput testing of molecules under development early in the drug discovery process.
These studies were performed using PCR-SSP, a protocol that is not amenable to high throughput testing.
Pressure of high throughput tests, particularly in a clinical setting, can lead to short cuts being taken during PCR set up.
There currently exists no technique to apply strain to many targeted individual cells in a larger culture in order to perform parallelized high throughput testing.
In order to facilitate high throughput testing for retrospective studies we have developed a capture probe based next generation sequencing assay for the detection of Gamma block SNPs.
Other key areas of research discussed in this chapter include the development of stem cell bioprocessing systems and microfluidic 'lung-on-a-chip' devices that provide a novel platform for high throughput testing of pharmaceuticals.
The results obtained with the two assays were in agreement but the improved plaque reduction virus neutralization assay was faster, more suitable for high throughput testing, and 10-fold more sensitive than the conventional virus neutralization assay.
Cell aggregates, or spheroids, have been used as building blocks to fabricate scaffold-free tissues that can closely mimic the native three-dimensional in vivo environment for broad applications including regenerative medicine and high throughput testing of drugs.
More suggestions(14)
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