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Three instrumental setups were tested to find the most economical, sensitive and high throughput setup.
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Although not tested here, SINBAD should be adaptable for high-throughput setups using large-scale magnetic separation systems.
Among the five strategies, SPE is the method of choice for analyzing larger sample cohorts as it can be adapted to high-throughput setups [ 34].
An additional fact which counter argues the "study bias" for these proteins is that, apart from various targeted small-scale experiments, many of their direct interactions have also been detected in independent high-throughput setups.
We investigated the detection of light in heat-induced transformants using a digital camera and a light-sensitive film in order to optimize the assay for high-throughput setups.
This means that up to 20 different markers can be used to characterize the cells in a sample, and high-throughput setups with 96- or 384-well plates will generate large numbers of data sets, each potentially comprising 1 2 million events in 10 20 dimensions.
Due to the high throughput of different setups, assays were carried out as whole cell biotransformations and samples were taken after 0 and 24 h. Figure 4 shows the OD600 values during cultivation as well as the maximum growth rates of the cultures for both enzymes at every tested setup.
The determined sensitivity of the analysis, the possibility to perform this metabolite analysis in high throughput, and the experimental setup would allow sampling of metabolites every 10 min to study time-dependent metabolite abundances.
This genotyping system consists of three major components: an automation system for high throughput DNA extraction and PCR setup, a conventional thermal cycler, and a LightTyper instrument for post-PCR melting temperature analysis.
Automation of data acquisition and data processing led to a fast and reliable high-throughput screening setup.
A new high-throughput analysis setup has been developed to investigate the acidity of up to eight solid materials in a parallel way.
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