Data acquisition occurred in a walk-away mode via the use of a high throughput sampling device.
In recent years, next-generation sequencing (NGS) technologies, which enable high throughput sample processing at relatively lower costs, are adopted in both research and clinical settings.
Cells were loaded onto the FACSCalibur using the BD Biosciences high throughput sampler (HTS).
A High Throughput Sample Instrument (HTS; BD Biosciences) was used to collect samples from 96-well plates.
Data was acquired using FACS DIVA software (BD) on an LSR II Flow Cytometer (BD) equipped with a high throughput sampler (HTS) and gated using FlowJo (Treestar).
Approaches such as immunomagnetic capture circumvent the need for cultivation but are currently neither reliable nor suited to high throughput sample screening [15].
Cytometry was carried out with a BD FacsCalibur cytometer fitted with a 96 well High Throughput Sampler.
In addition, the arrays are inexpensive and can be adapted to high throughput sample processing.
High throughput sample fractionation by tandem Equalizer beads (EB) and Mercapto ethyl pyridine (MEP) beads was used.
This suggests that an approach like QMC-PCR could reduce the need for highly purified DNA from high throughput sample preparation.
Alternatively, ELISA is a rapid, specific and sensitive method that has been used for surveillance purposes to enable high throughput sample screening.
