Sentence examples for high throughput qPCR from inspiring English sources

(F ) Heatmap of single cell high throughput qPCR immunoprofiling.

For gene expression in uterus, the 96.96 Dynamic Arrays for the microfluidic BioMark system (Fluidigm Corporation, CA, USA) were used to study by high throughput qPCR the gene expression profile in 6.5 ng cDNA from each sample, as described previously (Abot et al, 2013).

In this study, we evaluated the miRNA expression levels in FFPE and FF samples as well as several lung cancer cell lines employing a high throughput qPCR-based microfluidic technology.

The recently developed Microfluidic technology enables a significantly higher throughput qPCR analysis for a large number of samples; and assays in a much shorter time and at a lower cost compared to the conventional methods [ 17].

This study provides validation and guidance for the application of high-throughput qPCR workflows for gene expression profiling of single cells.

In a very recent study, Yuan et al. used a high-throughput qPCR study to screen 60,000 compounds and identified a small molecule capable of inducing PDX1 expression in the human PANC-1 ductal carcinoma cell line[68].

Custom high-throughput QPCR arrays were utilized to assay 128 genes known to be involved in osteoclastogenesis using the ABI 7900 HT system (Applied Biosystem, Foster City, CA).

Efficient data processing is required for the use of high-throughput qPCR applications.

Therefore, culture-independent methods like metagenomics and high-throughput qPCR may be rewarding in the future investigation.

Additional file 12: A table presenting overview of RNA extraction, cDNA synthesis, specific target amplification and high-throughput qPCR procedures.

Curcumin and its analogue induced expression changes in apoptotic and cell cycle related genes as determined by high-throughput QPCR.