Exact(1)
The results of these modifications were significant reduction in cost, compatibility with a variety of blunt-end restriction enzymes, and a streamlined protocol that was adaptable to high throughput population genomic applications.
Similar(59)
Examination of the number of reads of each signature revealed all the length or sequence variations found in the high throughput populations for all miRNA family members.
This method represents a reliable and low-cost alternative for detection of the common 22q11.2 microdeletions and can be adapted to high-throughput population screening.
These results, thus, support high-throughput population studies on mtDNA variation as another potential use of 454 sequencing [12], [13].
Hence, high-throughput population genomic studies aimed at detecting adaptive differentiation in marine fishes are rare, especially those employing a comprehensive sampling scheme.
To unveil the mechanisms behind the heterogeneity of population-specific responses would require the application of high-throughput population genomic approaches targeting either candidate genes of known function or genomic regions under selection combined with translocation/common garden and exposure experiments (Stinchcombe and Hoekstra 2008; Larsen et al. 2012).
Although this kind of heterogeneous divergence has also been demonstrated in other studies of marine fish populations [ 75], to date very few such studies have employed high-throughput population genomic approaches – nor have they had access to reference genomes – to characterize the genomic architecture of adaptation (but see [ 28- 31, 58]).
New high-throughput, population-based methods and next-generation sequencing capabilities hold great promise in the quest for common and rare variant discovery and in the search for "missing heritability".
As the method is rapid, inexpensive and amenable to high throughput, its applicability to population studies has been investigated in populations of swordfish, Xiphias gladius.
In less complex organisms like bacteria or yeast, propagation is straightforward and selection and phenotyping are often partly automated, enabling high throughput and very large population sizes (Wang et al. 2014; Jang et al. 2014; Nicoloff et al. 2007; Wiser et al. 2013).
Novel mutants having useful mutation in genes can be found by screening the population through high throughput ADP (Allele Discovery Platform) technique as mentioned in this article.
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