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We implemented a novel sonoporation gene transfection system using an aqueous two phase system (ATPS) for efficient use of reagents and high throughput operation.
In high throughput operation, we achieved a cost reduction of more than 50% compared with cost-optimized Sanger sequencing.
Although we confirmed that FLSSM is amenable to robotic high throughput operation using an automated cell picker such as the CellCelector™, this type of instrument is not essential to isolate high producers on a smaller scale.
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However, the ideas are general and can be applied to many other types of high throughput operations.
We have shown the value of tracking multi-step high throughput operations by using this knowledge to detect and correct misidentified data on gene expression microarrays.
We show the value of tracking multi-step high throughput operations by using this knowledge to detect and correct misidentified data on gene expression microarrays.
Multiple ePBR units can be networked to perform parallel experiments, enabling semi-high throughput operations.
In the recent past, an ever-growing market demand of drugs has led to the production of large batch volumes, which has put an enormous pressure on downstream processing for including higher throughput operations than conventional packed bed chromatography for rapid product isolation (Levine, 2002; Przybycien and Pujar, 2004).
Vertical channels (silicon-based through-holes), instead of conventional planar ones, were employed to achieve a higher density of sensing channels for a high-throughput operation.
However, the RP method was found to be more precise, more accurate, and, more importantly, more rugged than the HILIC method for maintaining the analytes' peak shape symmetry in high-throughput operation.
Unattended operation of the instrument revealed exceptional overall performance: Reproducibility (1 5% deviation in uncorrected elution times), repeatability (<20% deviation in detected abundances for more abundant peptides from the same aliquot analyzed a few weeks apart), and robustness (high-throughput operation for 5 months without significant downtime).
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CEO of Professional Science Editing for Scientists @ prosciediting.com