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It is anticipated that the protocols described herein will assist in the fast, high throughput monitoring of autophagy that can support other existing autophagy assays.
The flexible nature of such cell-free expression systems, when coupled with high throughput monitoring, can be especially suitable for protein engineering studies, allowing one to bypass multiple steps typically required using conventional in vivo protein expression.
It is labour-intensive, requires a high level of operator skills, and requires additional steps to positively identify CEC, which make them unsuitable for high throughput monitoring.
In addition, recent progress with a high throughput monitoring system allowed us to reveal naturally occurring variations in metabolite profiles in plant and this approach could also be a target for QTL mapping in Arabidopsis and tomato.
High throughput monitoring of functional status of cell subsets in peripheral blood based on whole cell MALDI-TOF MS could provide unique opportunities to monitor disease progression and resolution in clinical settings.
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"Approaches to high-throughput monitoring of antibody oxidation for process characterization studies: regional, peptide and site-specific" J. Hu, L.C. Vampola, A. Hilderbrand, J.W. Eschelbach.
Here, we describe a FRET-based multi-parameter imaging platform (FMIP) that allows simultaneous high-throughput monitoring of multiple signaling pathways.
Further, non-destructive measurements of material properties can enable in situ and high-throughput monitoring for applications including modulating cellular interactions.
The method also holds great potential for high-throughput monitoring the variation of estrogen levels in complex biological fluids, which is crucial for investigation of the molecular basis of various estrogen-involved processes.
The reduced manual labor and simplicity of operation of the SPR biosensor compared to ELISA, ease of sample extraction and superior real time semi-quantitative analysis are key features that could make this technology applicable in a high-throughput monitoring unit.
Additionally, bombardment was chosen over the traditional method of microinjection to allow for high-throughput monitoring of beads in cells.
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