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The overall analytical procedure is accurate and reproducible and accommodates high throughput; it is suitable for chemical quantification of a large number of RFH samples.
While the Nile Red screening strategy has the advantage of being high throughput, it is possible that some interesting strains could be missed due to the fact that cell wall composition, cell size and trophic levels in the sample negatively influence the number of positive hits (Cirulis et al.2012).2012
For high throughput, it is advisable to employ fast analyses using the 50-mm column, thus exploiting the high PPR at short analysis time.
First, the modelling shows that due to the high throughput it becomes experimentally challenging to change locally the auxin content of a specific cell without also dramatically modifying its transport properties, with all its possible consequences.
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In addition, in this simulation scenario, SAFARI achieves the highest throughput, it can provide consistently twice as much throughputs than the other two models, which achieve throughput of same magnitude.
Thus, the CaBChip is truly high-throughput: it employs nano-scale cultures, enables rapid and easy handling, is amenable to automation and fully compatible with standard microarray technology and equipment.
While our new method is quantitative and high-throughput, it requires, similar to the prevailing chromatin IP (ChIP) technique, a large amount of DNA and provides only average values for a given cell population.
Combined with high throughput sequencing, it will allow for in-depth analysis of large numbers of samples.
NFRA provides high throughput, but it is not suitable to low-cost devices.
When developing the high throughput method it was observed that there are differences in sensitivity of accessions to the herbicide (Al-Shugeairy et al. 2014).
For high throughput approaches, it may be necessary to accumulate many plates to process them simultaneously.
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