These evaluations are also applicable for high throughput investigation of protein stability.
Although local studies of flagellar functions have used the yeast two-hybrid approach to interrogate specific protein-protein interactions [23], [24], [25], [26], the utility of this technique for high throughput investigation has yet to be exploited for this organelle.
In spite of all these resources no HD genotyping array is yet available for this species that can facilitate high throughput investigation of many individuals for research and commercial breeding.
Advances in sequencing technologies have opened a new era of high throughput investigations.
Critically, the miniaturization attained enables high-throughput investigation of rare cell populations by reducing the requirement for cells and reagents by many-fold, facilitating advances in personalized vaccines which target DCs in vivo.
Improved screening techniques will facilitate this type of high-throughput investigation in zebrafish, adding to existing mammalian models as we advance our understanding of cardiovascular disease.
Moreover, employing our method with emerging technologies such as micro-fluidic devices or "lab-on-chip" will enable high-throughput investigation of multi-drug combinations, and become a promising platform for developing personalized medicine.
The rationale of discovery-based high-throughput investigation of disease is that there are molecular signatures (composed of genes, transcripts, proteins, and small molecules) that can be identified for better diagnosis, prognosis, and/or treatment of disease.
Whilst the 27K Infinium assay enables high-throughput investigation of individual CpG sites with high resolution, and is a considerable improvement on the previous Golden-Gate methylation assay, this methodology still has some limitations.
In the past 2 years, fully automated imaging techniques for the high-throughput investigation of plant root characteristics (yield determinants) have been developed [ 14- 16] to analyse non-destructively phenotypic traits such as root average radius, area, maximum horizontal width, and length distribution.
Pipelining the retrieval, the integration and the high-throughput investigation of such data in a fashion specifically tailored to the interests of the user should facilitate wider application by putting EST data in the hands of researchers directly addressing focused biological questions, without requiring the involvement of bioinformaticians.
