Gene Ontology (GO) analysis showed that high-ranking miRNA target genes were involved in metabolic processes, responses to stress, and signaling, indicating that these high temperature-responsive miRNAs have functions in diverse gene regulatory networks.
To examine if high temperature-responsive miRNAs can regulate the expression of their target genes, the expression levels of selected miRNAs and their target mRNAs were analyzed from tip and base tissues of pear shoots.
Results from deep sequencing and real-time PCR demonstrated that 37 °C high temperature-responsive miR397a and miR3627-5p were up-regulated in the shoot meristem tip tissue, while miR477b, novel262, novel177, novel345, novel188, novel197, and novel241 were down-regulated in response to 37 °C treatment (Fig. 5a, b).
Furthermore, unlike previous transcriptomic [ 26] and proteomic [ 4, 9, 27] studies, in our study 49 TDFs were identified as high-temperature-responsive genes.
Herein, we report a general and convenient approach for the preparation of pH or temperature or simultaneous pH and temperature responsive high flux and low fouling polyvinylidene fluoride (PVDF /copolymer blend ultrafiltration membranes by conventional phase inversion process.
TRPV1 is a nociceptive cationic (mainly Ca2+) channel responsive to high temperature (>43°C) (Boillat et al., 2014).
Significant numbers of csRNAs are responsive to high temperature.
Many of these were responsive to high temperature, drought or both stresses.
A glycine rich-RNA binding protein from rice is responsive to high temperature stress, and its over-expression disrupts thermotolerance in wild type yeast cells [ 53].
Our results revealed that HSP90, HSP70 and HSP20 in these differentially expressed genes (DEGs) were positively responsive to high temperature (Additional file 15: Table S5).
