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In order to avoid any potential spurious result in our association replications, the most conservative Bonferroni correction was used to ensure a high stringent condition for any positive result.
By using this high stringent threshold we tried to avoid false positives.
Hybridizations were performed under high stringent conditions using standard procedure [ 38, 39].
Hybridizations were performed under the high stringent conditions using standard procedure [ 26, 42].
Our results indicated that repeat masking and high stringent filter criteria could significantly decrease both FDR and FNR.
The persister cells with high stringent response regulator levels are known to have slow growth rates [ 21, 22, 28, 29].
The high stringent condition was chosen to achieve a more complete isolation of individual paralogues, orthologues, and homoeologues compared with using a low stringent condition.
It was observed that high stringent cut-off missed out either the right candidate and valid annotations and sometimes no hit was reported.
The genome sequence contigs that were "mapped" to LG8 were filtered with high stringent bit scores ≥ 400 to ensure the identification of true homologous sequences.
Next, p values were calculated using Fisher's exact test for all putative mutation sites, based on the distribution of read support with high stringent specificity and sensitivity [ 11].
Post hybridization washes were done in 2× SSC at 37°C (low stringent condition) and then at 60°C in 0.1× SSC (under high stringent condition).
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