Sentence examples for high stringency applications from inspiring English sources

A RIN of greater than or equal to seven indicates the RNA is suitable for high stringency applications [ 7].

We detected the CHEK2*1100delC mutation by application of diluted PCR products to nylon filters and hybridisation under a high stringency of [P]-labelled oligonucleotides complementary to CHEK2*1100delC and the wild-type sequence (5′-TTAGATTATGATTTTGGG-3′ and 5′-TTAGATTACTGATTTTGG-3′, respectively).

The membrane was subjected to a series of stringency washes (high stringency at 56°C) to eliminate nonspecific probe binding.

Washing was conducted in PBS/tween-20 with one high stringency wash at 57°C.

A high stringency protocol, suitable for systematic purification of polyclonal antibodies, is described.

All of the 22 tested T0 transgenic lines showed HptII banding after the high stringency hygromycin selection (Fig. 2b).

Overall, engineering arginines and phenylalanines for increased pKa and hydrogen bonding improved enzyme catalytic efficiency at high stringency conditions.

Low stringency washes in 2× SSC/0.1 % SDS for 15 min at 37 °C were performed twice, followed by high stringency washes using 0.2× SSC/0.1 % SDS at 68 °C, following the DIG Application Manual from Roche (South San Francisco, CA, USA).

Amplification was performed in a thermal cycler (Appendorf) using a high stringency PCR protocol with high annealing temperature to ensure specificity of amplification.

Solution 3 (high stringency) contained 0.1× SSC.

We used this high stringency to minimize false positive findings.