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This protocol provides a massively parallel pipeline to achieve high stringency and throughput.
A high stringency protocol, suitable for systematic purification of polyclonal antibodies, is described.
Indirect associations are removed by high stringency washes under denaturing conditions.
The membrane was subjected to a series of stringency washes (high stringency at 56°C) to eliminate nonspecific probe binding.
All of the 22 tested T0 transgenic lines showed HptII banding after the high stringency hygromycin selection (Fig. 2b).
Solution 3 (high stringency) contained 0.1× SSC.
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Washing was conducted in PBS/tween-20 with one high stringency wash at 57°C.
high-stringency cycle.
Following high-stringency hybridization and washing, all the blots were exposed to X-ray film.
We chose to further analyse the genes obtained by the high-stringency requirements.
The membranes were washed at 68°C under high-stringency conditions.
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