Additionally, in SGJS47, improvement in free fatty acid (FFA) composition, high-specificity production of short-chain fatty acids (C8, C10) and unsaturated fatty acids (C16:1) was achieved in crude glycerol medium condition.
By employing thioesterases BTE and 'TesA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450BM3 to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved.
Altogether, this work presents a promising strategy for high-specificity production of two excellent biofuels, isoprenol and prenol.
To our knowledge, this is the first successful report on high-specificity production of isoprenol and prenol from renewable sugar.
In this study, we present a microbial system for high-specificity production of isoprenol and prenol, two promising new generation of biofuels, from sugar feedstocks.
To the best of our knowledge, this is the first successful report on high-specificity production of isoprenol and prenol by microbial fermentation.
However, thus far, no study has been carried out for high-specificity production of isoprenol and prenol in either wild-type or engineered microbes.
The challenges met here are likely to be characteristic for high-specificity production of any isomers or homologues biologically, as these compounds are quite similar in structure.
The desired isoprenol and prenol were finally produced with high specificities, being the first successful report on high-specificity production of isoprenol and prenol from renewable sugar.
Specifically, peptide ligands benefit from their small size, easy and affordable production, high specificity, and remarkable flexibility regarding their sequence and conjugation possibilities.
Already shown to be a clinically viable means of reducing gene expression in a number of other diseases, therapies based on RNA interference (RNAi) can directly target hepatitis B virus transcripts with high specificity, profoundly reducing the production of viral proteins.
