This results in high time resolution and low frequency resolution at small scale and high frequency resolution and low time resolution at large scale.
In order to achieve high specificity (and also resolution), methods that allow covalent capture of RNA protein interactions in vivo have been developed.
Fluorescence microscopy is a widely-used imaging method owing to its high specificity and minimal perturbation, yet its spatial resolution had been classically limited to 200 300 nm by diffraction of light.
With the advent of single-molecule localization microscopy (SMLM) techniques, intracellular proteins can be imaged at unprecedented resolution with high specificity and contrast.
Non-optical imaging techniques (high resolution 1(H) Nuclear Magnetic Resonance Spectroscopy) have been suggested for clinical diagnosis of Acanthamoeba keratitis, analyzing its biomechanical profile, and have proved able to identify Acanthamoeba isolates with high specificity and sensitivity [ 18].
Hence, by combining photoacoustic imaging with molecular imaging (i.e., photoacoustic molecular imaging), brain glioma can be diagnosed with high sensitivity, specificity, and resolution at greater depth [18, 19, 20].
Novel imaging methods with high sensitivity, specificity, and resolution at tumor-relative imaging depth are urgently needed for accurate diagnosis of glioma, based on which precise tumor surgery can be performed to achieve complete tumor removal and improve the prognosis of the patients [5, 6].
Second harmonic generation (SHG) microscopy is a recently developed technique which allows examination of fibrillar collagen structures with a high degree of specificity and resolution.
