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To the best of author's knowledge this is first report on high solid saccharification on rice straw without external supplementation of β-glucosidase.
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Also, soluble xylo-saccharides may change the distribution of water on the solids surface by lessening the water constraint at high solids saccharification [ 15].
The present study demonstrated that fed-batch hydrolysis resulted in higher solid saccharification with high saccharification yield.
The fiber fraction was subjected to high solid load saccharification (25% DM) to enhance the lignin content in the feed for subsequent organosolvation.
High solid enzymatic saccharification could solve this problem but has several other drawbacks such as low rate of reaction.
In addition, high solid enzymatic saccharification has been suggested to increase the initial conversion rate and final fermentable sugar concentrations [ 19], but can exacerbate enzyme inhibition and pose rheological challenges that must be taken into account.
Figure 3 shows that glucose and xylose yields in high solids enzymatic saccharification were significantly lower compared to enzymatic hydrolysis yields using washed low solids concentrations as shown in Figure 2.
In this context, the high solid loading on saccharification and fermentation and the use of whole-slurry from pretreatment could be promising alternatives to obtain high ethanol concentrations and to decrease operational costs and wastewater.
Enzymatic hydrolysis and high solids loading simultaneous saccharification and fermentation (SSF) results were also satisfying.
As a part of this enzymatic saccharification method, we generalized and expanded previously developed yield calculations that account for the multiphase nature of the high-solids enzymatic saccharification slurry.
Many researchers have tried different mixing mechanisms to get better saccharification yield at high solid loads.
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