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As described above, T242X had a high reversion rate.
Conversely, it is also plausible that an amino acid change with a high reversion rate, presumably with a functional or structural constraint at that position, is unlikely to accumulate rapidly in the population.
This also illustrates the high reversion rate, as 80% of cells do not survive a re-plating on 40 μg/ml nalidixic acid.
Similarly, another study from India reported a higher QFT-GIT test conversion rate compared to reversions [ 16]; however, they also reported a high reversion rate unlike our findings.
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This is supported by high reversion rates of escape mutations selected by 'beneficial' HLA Class I alleles following transmission to HLA-mismatched recipients [22][23].
As we were not able to identify the other ins mutations by complementation experiments due to high reversion rates, we looked at phenotypic analogy with mutations described in the litterature.
However, studies from The Gambia [ 24], Japan [ 25] and South Korea [ 26] reported high reversion rates following treatment completion.
We observed that the high frequency low level amp resistance was indeed extremely unstable, showing very high reversion rates to antibiotic sensitivity.
Through such successive exposures to increasing antibiotic concentrations it was possible to evolve E. coli that survived on amp concentrations as high as 10 μg/ml, yet showed very high reversion rates, indicating the absence of stable mutations conferring resistance.
This class of theoretical models (Ishii et al. 1989; Travis and Travis 2002) assumes that alleles mutate back and forth between defined states that are most beneficial in either of two different environments (i.e. high reversion rates, with an absence of unconditionally deleterious alleles).
Patients with Δ t < 48 hours showed a higher reversion rate of AF in the ER (P = 0.009).
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