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Our study suggests that the DBS collection method is efficient, yields a high response proportion and blood spots that can be used in large population-based studies.
The population-based sampling together with a high response proportion compared to other German cohorts [ 51] limit major selection bias and increases the external validity of these results.
It did presumably contribute to a high response proportion of 29%; much higher than would normally be expected from a mail-out survey.
Strengths of the current study include the large sample size, high response proportion (91% of cases and 81% of controls), and that cases and controls were drawn from the same well-defined population (McGlynn et al, 2007).
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Studies have demonstrated higher complete response proportions in patients who receive more than six induction cycles of methotrexate-based chemotherapy [ 17, 31].
External validity is not solely dependent on the response proportion; high response rate constitutes an enhancing factor, although it is not necessarily associated with an unbiased estimation of study outcome.
Although the response proportion was high, approximately a third did not participate.
Moreover, the high response rates for NHANES (the proportion of sampled individuals who were interviewed and had a physical examination was 76% in 2003 4 and 77% in 2005 6) indicate that obesity-dependent selection bias is unlikely.
The major strengths of our study were its prospective design, its high response rate, and the negligible proportion of losses to follow-up.
Again, accuracy was very high with correct response proportions of 99% in both conditions (±2 for the central square and ±3 for the four squares).
The major strengths of our study are its prospective design, high response rate (80 %), and negligible proportion of loss to follow-up (0.3 %).
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