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For further resolution of the patient's DR and DQ typings we performed NGS high resolution typing using Illumina TruSight v2 sequencing panel.
Both methods are very useful for clinical and research purposes and can provide generic (low resolution) to allelic (high resolution) typing results [24].
The number of small units repetitions varies in different individuals, making thus SSRs good markers for the high resolution typing of individuals27.
The aim was to evaluate the use of combination of SBT (sequence based typing) and HARP (heterozygous ambiguity resolving primer) in HLA typing to acquire high resolution typing results.
High resolution typing was achieved using reference strand conformational analysis (RSCA).
HLA-DRB1* loci typing among those replication cohort specimens that were positive for DRB1*15, as well as high resolution typing of HLA-DRB1*15 per se were similarly performed by PCR-SSP.
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A total of 1281 recipients of fully HLA-A, B, C, DRB1, DQB1, DPA1, DPB1 high resolution typed UD-HCTs were included in the analysis.
It is important to note that another role for our high resolution typed archive is to confirm the existence of reported rare HLA alleles.
Lastly, the smallest set of ultraspecific probes is selected such that the highest resolution typing can be achieved with a minimal cost in producing the assay.
Lastly, the minimum number of these ultraspecific probes is selected such that the highest resolution typing can be achieved for the minimal cost of production.
When a high-resolution typing approach based on loci with fast mutation rates, such as MLVA, is used, near matches should be the rule rather than the exception.
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