9 High-resolution slide images from each H&E, α-SMA, and Azan stained section were obtained using a Nano Zoomer 2.0-HT slide scanner (Hamamatsu Photonics, Hamamatsu, Japan).
Briefly, seamless high-resolution slide images were acquired using the Scanscope FL 10-bit monochrome TDI line-image capture camera using filters specific for DAPI to define the nuclear compartment, fluoroscein isothiocyanate (FITC) to define cytokeratin for the tumor cytosolic compartment, Cy3 to define the target marker ATM, and Cy5 to define vimentin-positive nonmalignant stromal cells.
The method consists of a modified version of the well-known, parametric, high-resolution, sliding window estimation of signal parameters by rotational invariance technique (ESPRIT) method.
Stained sections were scanned with a high resolution, whole slide imaging system (0.46 μm/pixel with 20X objective lens, ScanScope™ XT, Aperio Technologies, Inc. Vista, CA, USA).
Once mounted, slides were scanned with a digital scanner NanoZoomer (HAMAMATSU, Réf: 2.0-RS: C10730-13) tobtainin high resolution virtual slides.
By imaging large numbers of slides automatically at high resolution, modern automated whole slide imaging (WSI) systems have the potential to become useful tools in pathology practice.
Coupling of these matrix features with the 10 ms-high temporal resolution sliding window has enabled us to look into the real-time transient dynamics associated with memory acquisition and recall in the following ways: First, CA1 ensemble patterns evoked by the CS-US pairing change rapidly from unconditioned forms to conditioned associative forms.
The platform involves using automated equipment to handle large numbers of PCR plates, small nitrocellulose slides, high resolution scanners, and the screening of several rounds of pools.
To quantify poly(GP) pathology in the cerebellum, high resolution images of immunostained slides were captured using an Aperio ScanScope XT scanner and analyzed using ImageScope software (Leica Biosystems, Buffalo Grove, IL).
This high-throughput approach would not have been practical at the time when some tumor antigen screening methods such as SEREX were first developed since this new approach makes use of automated equipment for handling large numbers of PCR plates, small nitrocellulose slides, high resolution scanners, and accurate printing machinery.
For example, studies of case scenarios with high resolution pictures or computer slides representing real or simulated blood loss can be used to replace the simulated blood loss stations, reducing the need for consumables and expensive teaching or assessment methods [ 13].
