Of the 251 putative anchor genes selected, 200 were analysed using high resolution section in situ hybridisation (SISH) of paraffin-embedded E15.5 murine kidney.
In the study presented here, we have extended the analysis of the kidney development gene atlas using high resolution section in situ hybridisation (SISH).
Although some high resolution section analyses with mouse tissues are not sufficient for providing global views, high similarities in the expressions of the orthologous genes in zebrafish and mouse were observed (Table S2).
Here, this data is further analysed to identify anchor genes via stringent bioinformatic filtering followed by high resolution section in situ hybridisation performed on 200 transcripts selected as specific to one of 11 anatomical compartments within the midgestation mouse kidney.
Full clinical evaluation with pulmonary function studies (with flow volume loop), computed tomography (CT) scanning with high-resolution sections, and bronchoscopy can help differentiate these disease states.
The continuous high-resolution sections of aeolian dust deposits inter-bedded with fossil soils from the Priobie Loess Plateau and the Northern Altai Plains – mapped as a part of the 2000 km W E Siberian palaeoclimate transect – have produced extraordinary evidence of the Late Quaternary climate evolution and landscape development in the parkland-steppe north of the Altai-Sayan Mountains. Altai-Sayan Mountains
The thermostability of SYNβglu allows it to be visualized in high-resolution sections from paraffin wax embedded tissues.
The eMouseAtlas IIP3D viewer employed for the display of the high-resolution sections was initially developed within the group (8) as a means of overcoming the issue of delivering very large tiled or 3D images within the constraints of a web browser.
So far we have been concerned with the energy decay rate from the largest scales, which we have shown to be well captured by the ILES (Section 4.1), and with the energy transfer in the inertial range, which we have seen to be described accurately only at much higher resolutions (Section 4.2).
Scanning Electron Microscopy (SEM) imaging of the MO nanoparticles (NPs) was performed in an FEI Quanta 200 environmental, while high resolution cross-section images of the samples was performed in a Hitachi HD2300 transmission electron microscope (TEM) operating at 200 kV.
After digital recording of these fluorescence data encompassing the entire specimen at a high resolution, the same sections were stained with Klüver-Barrera method to obtain their exact bright-field counterparts.
