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High resolution objective lenses have high numerical apertures and low working distances (100 200µm), as they are developed for investigations of slice preparations.
High-resolution objective lenses, meanwhile, are now being made at very high cost using aspheric lenses.
To meet the requirement of extensive temperature adaptability of infrared optical systems, diffractive optics is applied to a high resolution Petzval objective in the 3-5 μm waveband and a hybrid Petzval objective, which is passively athermalised over the temperature range −20 °C to +50 °C, is designed.
It has been a challenge to overcome the corneal curvature radius to design a full-pupil field, non-contact and high resolution corneal curved objective lens, which covers the cornea full-pupil field and has the ability to resolve corneal cells.
We have used lensfree digital holography in cost-effective and robust devices for a range of applications to capture images with a high space-bandwidth product, i.e., images that simultaneously have a very large field of view (>20 30 mm) and a high resolution (equivalent to microscope objectives with NA as high as 1.4).
Analysis of the mitochondria from images taken at higher resolution (63× objective) also supported the length-dependent shift in the control group.
For high resolution imaging, a water immersion objective lens (Olympus UPLSAPO60XWIR 60X/1.20) is used.
In this paper, we report an optical design of a full-pupil field, non-contact corneal curved objective lens for high resolution cornea imaging.
Based on earlier work on IDIFs [18, 20, 21] and given the high resolution of the HRRT, an additional objective was to assess whether a carotid artery-based IDIF could be used as a noninvasive alternative for arterial sampling in the case of both CBF and CMRglu measurements, thereby increasing clinical applicability of this methodology in humans.
Therefore objective assessment by high resolution automated activity recording seems mandatory.
Third instar larval filet preparations immunostained for Ret and integrins were analyzed by confocal microscopy with high resolution using a high NA oil objective (Zeiss LSM700, 40×/NA1.3, z step size: 300 nm).
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