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PCR products were separated on 2.5% high resolution Metaphor agarose gels (Lonza) stained with ethidium bromide for visualization.
Microsatellites were run on 4% high resolution MetaPhor agarose gels for 2 h at 78 V/cm, stained with ethidium bromide (0.1 μL/mL), and photographed under UV light using an Alpha Imager 2200.
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The resulting 100 bp PCR fragments were separated on 4% high resolution agarose gels (Metaphor Agarose, Lonza), purified using a QIAquick gel extraction kit (QIAGEN), eluted in 30 μl 10mM Tris-HCl pH 8.5 (EB buffer) and dried down to a volume of 15 μL using a SpeedVac.
Electrophoretic size separation of SSR PCR products was performed in 3 % MetaPhor (Cambrex) high resolution agarose gels in 0.5 fold TBE (45 mM Tris-borate, 1 mM EDTA, pH 8.0).
SSR PCR products were size separated in 3 % MetaPhor (Cambrex Corporation, East Rutherford, USA) high resolution agarose by gel electrophoresis in 0.5 fold TBE (45 mM Tris-borate, 1 mM EDTA, pH 8.0) and visualized by ethidium bromide staining.
High resolution only.
High resolution image is inserted.
floor at a high resolution.
Low mag, high resolution optogenetics.
NIN is very high resolution, detailed noise.
A comparative high resolution endostructural assessment.
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