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High resolution fluorescence imaging was carried out by confocal microscopy (Leica TCS-SP5 AOBS).
allowed documentation of high resolution fluorescence images.
It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans.
We further investigated presence and localization of vGLUT1 and vGLUT2 protein in gray matter NG2 cells in hippocampal slices by applying high resolution fluorescence microscopy, subsequent to patch-clamp recording and biocytin filling.
High resolution fluorescence microscopy was performed with Zeiss Axio Imager.Z1 microscope equipped with a Zeiss Apotome and AxioCamMRm CCD camera.
During autophagasome formation, GFP-LC3B is processed and recruited to the autophagosome membrane where it can be imaged as cytoplasmic puncta by high resolution fluorescence microscopy.
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Higher resolution fluorescence mapping could one day be used to help assess crop yields and how they respond to drought and heat in a changing climate.
Cryo-phase contrast images have poor resolution, but they can be used to estimate ice thickness (Sartori et al. 2007) and to direct (higher resolution) fluorescence imaging only to regions where ice thickness is appropriate for cryo-ET, thus greatly reducing the number of images taken.
The migration of two different cell types was monitored for 72 h by high-resolution fluorescence imaging.
The thermal imaging of an active nucleation site was performed by utilizing high-resolution, fluorescence microscopy, which enabled a maximum spatial resolution of 370 nm/pixel.
Therefore we highlight several techniques that can be utilized to preserve both antibody and fluorophore-conjugated phalloidin labeling within Xenopus embryos for high-resolution fluorescence microscopy.
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