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Selected clones were used to perform high resolution FISH (Fluorescent In Situ Hybridization) mapping on giant meiotic lampbrush chromosomes, allowing meiotic mapping in addition to the confirmation of the order of the three clusters along the chromosome.
High resolution FISH on pachytene chromosomes or chromatin fibers using probes of pAv34 and the HinfI-satellite could be used to gain information about possible interspersion or physically neighborhood of both satellite families.
Our data confirm and refine the GGA16 chromosome organization, by using three integrated mapping strategies: RH mapping, genetic mapping and high resolution FISH, especially on lampbrush chromosomes from growing oocytes which were shown here to provide a unique opportunity to study recombination hot spots and to analyse their molecular context.
To confirm this organization and build precise genomic markers maps, we integrated three alternative mapping strategies, namely Radiation Hybrid (RH) mapping, genetic mapping, and high resolution FISH mapping on giant lampbrush chromosomes from growing oocytes, allowing the estimation of recombination rates along the chromosome.
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Despite its much higher resolution, FISH on interphase nuclei using a panel of probes covering the most frequent genomic inbalances seen in CLL still cannot replace classical cytogenetics, which is superior in detecting complex karyotypic alterations that are of prognostic impact.
This fact makes it possible to recognize the 22 bivalents of this species and therefore allows them to be mapped by high-resolution FISH.
To acquire high-resolution FISH images in a multi-spectral scanning mode, a huge amount of image data with the stack of the multiple three-dimensional (3-D) image slices is generated from a single specimen.
Our detailed RH map and high-resolution FISH mapping results on giant lamprush chromosomes from chicken oocytes clearly demonstrate a different order for the three complexe: centromere - NOR - Y - B. The orientation with respect to centromere is obvious both from FISH data in which the centromere is visible and from RH mapping data.
Such configuration further indicates that Tetris elements with expanded arrays accumulated close to the α-heterochromatin of chromosome 2. High-resolution FISH on DNA fibers (fiber-FISH) with the two probes described above showed several cases where multiple copies of Tetris are located in a genomic region corresponding to less than 100 kb (fig. 5 A and B).
FISH or Southern blot analyses were used to identify MLL rearrangements, more than three copies of RUNX1 by FISH define iAMP21, and high resolution SNP arrays and/or FISH were used to detect dic(9 20) aberrations [ 43, 44].
When used in line with the CUFES it provided high resolution maps of staged fish eggs and zooplanktonic functional groups.
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