Future developments may enable high-resolution expression of genome engineering proteins, which might increase HDR rates further, and may contribute to a better understanding of DNA repair pathways due to spatiotemporal control of DNA damage induction.
While studies of FAA in mice have become more prevalent, the conditions which foster high-resolution expression of FAA in mice have not been optimized.
Importantly, the high-resolution expression patterns presented here improve our understanding of the molecular mechanisms and expand our knowledge of reaction wood formation in Populus.
The high-resolution expression atlas generated here provides a systems-level overview of the epistatic landscape between GSTFs and reveals underlying mechanistic details.
Importantly, the high-resolution expression patterns presented here further our understanding of the molecular mechanisms involved in cold resistance and signal regulation for bulb flower breeding.
To address this question in a systematic way in vivo, we carried out high-resolution expression analysis of Drosophila TDP-43, TBPH, and show that similar to its human homologue, TBPH is expressed in nuclei of glia and muscle cells.
As the SuperGenome mapping is performed on single nucleotide level, high-resolution expression height graphs resulting from RNAseq or tiling array experiments can also be investigated within a common coordinate system, which is especially useful for comparative analyses.
In order to preserve the relationships among brain circuitry, cell type, and gene expression, all of which are crucial for understanding the molecular machinery of the brain, in situ hybridization technology has been developed [ 4], which can be applied to measure the three dimensional high-resolution expression map of brain genes one at a time.
Here, we use high-resolution expression profiling to identify three waves of gene activity: a post-fertilisation wave involving polyadenylation of maternal transcripts; a broad wave of zygotic transcription detectable as early as the seventh cleavage and extending beyond the MBT at the twelfth cleavage; and a shorter post-MBT wave of transcription that becomes apparent as development proceeds.
In recent years, high-resolution expression data measured in mammalian brains became available in quantities and qualities never witnessed before (Henry and Hohmann, 2012; Lein et al., 2007; Ng et al., 2009), calling for new ways to analyze neural gene expression images.
Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database.
