Using an array of high resolution assays to characterize a variety of discrete developmental processes, we show that the Macondo oil does not cause general and wide-spread toxicity but rather disrupts specific developmental processes, some of which have never been reported previously for any crude oil source.
Therefore, a higher resolution assay, such as genome sequencing, may identify more breakpoints and explain some of the discrepant results.
We anticipate that our high-resolution assay may help decipher the mechanisms and regulation of these helicases in the future.
Although BGS is a high-resolution assay, this technique is less sensitive than MSP as detection requires at least 25% of the alleles to be methylated [ 22], suggesting a risk of disagreement between the two methods.
As of September 2012, the TIARA database provides raw data and variant information for 13 sequenced whole genomes, 16 sequenced transcriptomes and 33 high resolution array assays.
Traditional bulk-based methods and high-resolution assays were employed to evaluate the physicochemical, functional, and bio-recognition attributes, as well as the in vivo toxicity profile of the active substances in Copaxone and Synthon EU FOGA lots.
The resulting data will make possible the development of very low cost and high-resolution assays for the systematic typing of any new isolate.
Polyadenylation reactions, product separation and visualization were performed as described for high-resolution assays.
Control DNA, used to validate the high resolution melt assay, was extracted from anonymized tumor specimens compiled by the frozen tumor bank, OvCaRe (Ovarian Cancer Research), under written informed consent.
Samples for the high resolution melt assay were purchased as either DNA or tissue blocks from vendors who provided unlinked anonymized specimens collected in accordance with applicable review boards approval, regulations and laws.
