In this case, the medicine is the high quality reading that comes at a much higher price than say, advice you give yourself for free or that a friend might give you.
For example, a very strong Sono-seq effect in a control sample is expected to give ChIP-seq libraries with high read clustering that is a combination of true ChIP (antibody-specific) signal plus Sono-seq-derived noise that covers promotors and enhancers in a nonspecific manner.
This is confirmed by results presented in the supplementary data: the data set D4* was mapped to the reference and quality filtered to ensure only high quality reads that stem from the actual organism with high probability.
The other reading, that high confidence means high uncertainty, next to being counter-intuitive, does not reflect that agreement sometimes does give an indication for the truth of a finding.
From a total of 145,836,644 raw reads, 102,505,713 (70%) were "high quality reads" that passed the Illumina filter while 120,438,739 (83%) contained the barcode and the cut site and no "Ns" within the first 72 bases and were not adapter/adapter dimers.
Illumina sequencing generated 54,248,006 high quality reads that were assembled into 83,580 unigenes with an average sequence length of 612 base pairs, including 13,315 clusters and 70,265 singletons.
In this study, a combination of RNA-seq and three DGE analyses were performed using Illumina sequencing, which generated 54,248,006 high quality reads that were assembled into 83580 unigenes with an average sequence length of 612 base pairs.
For each population the per individual average of raw read counts (Raw read count in Million bp), the number and percentage of high quality reads that were successfully aligned to the stickleback genome (Bowtie aligned), and the percentage of the aligned reads subsequently fed into Stacks (% Raw reads used) are presented.
At the time, many investors were unwilling to make any bets on the stock market, but those who relied on that high reading to invest in equities have been rewarded.
RNA-Seq generated approximately 100 M high-quality reads that were assembled into 69,393 unigenes (mean length = 853 bp), including 14,531 clusters and 34,194 singletons.
