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All three DEHP metabolites as well as the PFAAs had relatively high quantification rates.
Due to the high quantification accuracy, SILAC has become a versatile tool for multiple proteomic applications [ 123- 125].
For this, 4-plex iTRAQ (isobaric Tags for Relative and Absolute Quantification) is best suited to study four different samples in one single experiment with high quantification accuracy [ 15].
This circumstance is also substantiated by the fuzzy logic-based assessment which revealed quite a high score (0.75) for the fuzzy-logic indicator (see Table 4), associated with lower performance from the biscuit/Wizard DNA extraction combination (i.e. high quantification bias).
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Cy0 method resulted in higher quantification values, leading to the decrease of total variability in CASP3 and LYZ genes.
The highest quantification limit was found for hexanone which was the least abundant compound in chromatograms acquired during the calibration procedure.
Combustion IRMS and ferrographic enumeration had the lowest quantification limits (∼2 to 20 cells/ml), whereas microplate spectrofluorometry had the highest quantification limit (∼105 cells/ml).
Results from thirteen mobile phase compositions showed that the mobile phase composition of ACN/NaAcetate (5 mM /MeOH/Acetic acid (pH 3); (30:40:30; v/v/v) resulted in the highest quantification value for OTA.
The anorexic bacterial profile is similar to the lean control group for the Firmicutes, Bacteroidetes, and Lactobacillus but different for M. smithii (anorexic group had higher quantification).
The highest quantification of both sides of the lung was used to obtain the total bacterial score.
We have previously compared both methods obtaining higher quantification in wastewater samples when applying the first one [ 37].
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