Sentence examples for high quality total from inspiring English sources

The electrophoregram of a high quality total RNA sample consisted of two well-separated ribosomal peaks.

High quality total RNA was isolated using the RNAeasy™ (Qiagen) kit and flash frozen.

Following preparation of sufficiently high quality total RNA, samples were labelled, loaded onto the array slides and read following the manufacturer's instructions.

Briefly, 2 µg of high quality total RNA was reverse-transcribed using a T7 tagged oligo-dT primer for the first-strand cDNA synthesis reaction.

One µg of high quality total RNA was converted into double-stranded cDNA using the Affymetrix One-Cycle Target Labeling Kit using an oligo-dT primer containing a T7 RNA polymerase promoter.

High quality total RNA was prepared as described 33.

This modification produces high-quality total RNA suitable for RNA sequencing and microarray analysis.

First-strand cDNA was synthesized from 1 μg of high-quality total RNA using SuperScript II Reverse Transcriptase in accordance with the manufacturer's instructions (QIAGEN, Omniscript RT).

Calculation of fCO2 from high-quality total dissolved inorganic carbon (CT) and total alkalinity (AT) measurements does not yield results comparable in accuracy and precision to fCO2 measurements.

Cells were then washed in PBS and high-quality total RNA was extracted RNeasy Midi Kit, according to the manufacturer's instruction (Qiagen, Valencia, USA).

All samples have Abs 260/280 ∼2.0, Abs 260/230 >1.4 and Experion's RNA Quality Indicator (RQI) >7.0, indicating high-quality total RNA preparations and consistent extraction procedure (data not shown).