Exact(8)
Underpinning all of these important developments is the need for high quality tissue samples and the impact of pre-analytical variables on tissue research is discussed.
In addition to producing high quality tissue, this method vs use of cell lines also allows us to study tissues with intact stromal-epithelial interactions occurring in vivo.
The ability to access high quality tissue to perform these experiments was one of the limiting factors.
The importance of collecting high quality tissue from normal individuals outside of blood is an on-going issue.
Methods: With such a large sample of high quality tissue available for the analysis, why were scats (n=6) included in the analysis?
These studies lead toward the development of biobanks that are capable of providing well annotated high quality tissue samples that can meet the current demands of cancer research community.
Similar(52)
To ensure accurate genotypes of lower quality fecal DNA and to prevent contamination with higher quality tissue DNA, polymerase chain reactions (PCRs) for scat samples were performed on 96-well plates separately from those used to genotype tissue samples.
Higher quality tissue DNA, used for Southern blotting, was prepared using the Puregene® DNA Purification Kit (Gentra Systems, Minneapolis, MN, USA): 10 20 mg tissue was crushed in liquid nitrogen with a pestle and mortar and the DNA extracted according to the manufacturer's instructions.
The approach employed here—utilizing higher quality tissue and more precise dissection of brain regions than is usually possible in humans may therefore provide a powerful means to investigate variation in gene expression relevant to complex brain related traits, including human neuropsychiatric diseases.
Arthritis models are mainly developed in small animals while high-quality tissue engineering experiments ask for a large animal model.
When performed in realistic, high-quality tissue images, the conclusions derived from these approaches with regard to the levels of random spatial associations of sinusoids and CARcs are well in line with ours21.
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