Importantly, the design of the sample holder enabled the collection of high quality spectra that were not dominated by the δ(OH) mode of water.
The cell is inserted in a MCT-FT-IR, which allows to collect high quality spectra with a 1 s time-resolution.
A special focus is put on the application of in situ Quick-XAS that permits to acquire high quality spectra at the Ag K-edge with a good time resolution (30 s), thus informing in real time on the transformations of the silver species.
In addition, we demonstrated that freezing cells at −80°C was sufficient to obtain high quality spectra, whereas cell lysis increased background noise in a non-interpretable way.
Proteomic expression data is also available for many parasite species [4], [5], [6], [7] but coverage, especially when only high quality spectra are used, is generally much sparser than for microarray studies, thus limiting their usefulness.
However, in order to acquire high quality spectra for structure elucidation, the NMR method requires relatively large amounts of pure compound (up to 100 µg), often requiring substantial purification from extracts or other mixtures of compounds.
High quality spectra could be obtained more quickly for batches of larvae, extracted into a single droplet sample.
In contrast, high quality spectra were obtained for the vast majority of the 2% SDS and 70% FA extracted lysates.
Identifications tend to be more reliable when the sequence coverage is large or based on a few high quality spectra.
