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An average of 35595 high quality sequences per sample was obtained from 12 colonic content samples (Supplementary Table S1).
The qualitative and quantitative analyses performed on the ready-to-sequence libraries were a crucial step for achieving high quality sequences.
In order to obtain high quality sequences, tags with length of <220 nt, average quality score of <20, and tags containing >3 ambiguous bases were removed by PANDAseq.
All the high quality sequences were classified into 99 OTUs (97% similarity cutoff).
These high quality sequences had an average read of 500 bps.
The number of total high quality sequences obtained from raw pyrosequencing data was 4586 reads.
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High-quality sequences were used as input.
These numerical values represent that high-quality sequences were obtained for further analysis.
High-quality sequences were used for downstream analysis, and chimera sequences were removed with the UCHIME algorithm.
Following ITU P.910 recommendations [7], it is the comfortable distance according to high-quality sequences.
The size of high-quality sequences for each sample ranged from 23,554 (CFM-38) to 40,512 (CFM-98).
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