We have found that chilling fresh tissue prior to sectioning is essential for obtaining high quality sections.
Overall, the Compresstome and Vibratome both produced high quality sections from unfixed spleen, lymph node, vagina, cervix, and uterus, and subsequent immunofluorescent staining was equivalent.
To survive on this poor-quality diet, they have to eat the high-quality sections of the bamboo plant, such as the tender leaves and shoots, in large quantities, over 1.5 kg of fresh leaves and 4 kg of fresh shoots daily.
Resulting high-quality sections were then imaged for digital analysis at 0.42 μm/pixel with an Olympus Nanozoomer.
FIB-SEM has been used on biological tissues in two ways: (1) a sample may be embedded, sectioned, and imaged using the FIB-SEM apparatus (Heymann et al., 2006); or (2) in some cases, the FIB capability is used to produce high-quality sections for TEM imaging (Wei et al., 2012).
The gallium beam can be used for imaging, can mill an area of interest to serially expose surfaces to reveal tissue and cell features, or can be used to produce high-quality sections of a sample for transmission electron microscopy (TEM) studies.
These paraffin blocks provide high quality histologic sections, which are used for pathologic interpretation and assessment, and are mirror images of the frozen sections used for RNA extractions.
For the preparation of sediments and colloidal suspension in nonmarine, low ionic strength aqueous environments, Nanoplast embedding yields high quality ultrathin sections free of extraction artifacts and salt precipitation with minimal handling disturbance to the structural integrity.
This technology has been already employed in [17] in order to obtain high quality fiber cross section.
It includes the development of computational tools comprising both Monte-Carlo and deterministic transport, sensitivity and uncertainty codes, the generation of high quality neutron cross-section and covariance data libraries.
