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However, preparing high quality representative RNAs for sequencing to generate metatranscriptome is a challenge.
This is dependent on high quality, representative data, which is in large part gathered from national monitoring and evaluation (M&E) efforts.
The xenoarray approach does not directly address a key bottleneck for expression library-based screens, i.e. their reliance on high quality, representative cDNA collections and the ability to efficiently introduce these genes into mammalian cells.
High quality representative data within electronic patient records from primary care can be used to derive and validate robust risk prediction algorithms which can be then implemented and evaluated in clinical settings.
A total of 74,573, 54,913, and 104,739 assembled transcripts were recovered from P. axillaris, P. exserta and P. integrifolia, respectively and following removal of multiple isoforms, 32,994 P. axillaris, 30,225 P. exserta, and 33,540 P. integrifolia high quality representative transcripts were extracted for annotation and expression analysis.
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The highest quality representative in each subset is chosen using AEROSPACI scores, which provide a numeric estimate of the quality and precision of crystallographically determined structures.
Additional high-quality representative images were captured on a Leica SP2 confocal (Leica Microsystems, Buffalo Grove, IL).
However, in developing countries, primaries are frequently dominated by elites and conducted without transparency, decreasing the likelihood that parties select high-quality, representative candidates.
A large sample of high-quality, representative primary care data was available to test the updated algorithm.
The first high-quality, representative Wuzhishan miniature pig genomic BAC library had been constructed, covering about 7.68-fold genome equivalents of the porcine genome.
Numerous recent studies report continued assembly developments and comparisons, which reflects the importance of generating a high-quality, representative genome sequence (Bradnam et al., 2013; Powers et al., 2013).
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