Sentence examples for high quality paired from inspiring English sources
Only high quality paired and orphan reads were pooled for assembly.
BAC: bacterial artificial chromosome; BES: BAC end sequence; SBH: single BLAST hit; PBH: paired BLAST hit; hqPBH: high quality paired BLAST hit; lqPBH: low quality paired BLAST hit.
High quality (paired and unpaired) reads were assembled de-novo using SOAPdenovo assembler [ 31] independently at eight different K-mers (21, 27, 33, 39, 45, 51, 57, 63).
High quality paired reads (96.7-97.896.7-97.8%raw data) were mapped to the annotated equine genome (EquCab_2.71) [ 64] using bowtie2 [ 65] and gene transcription was inferred using RSEM version 1.2.11 [ 66].
The remaining high-quality paired sequences were used for de novo assembly using scaffolding and autodetection of paired distances with default mapping options.
A total of 6,710,574 high-quality paired end reads of 2×36 bp were generated.
We constructed MF and WGS libraries from S. moellendorffii and produced 1,621 and 1,598 high-quality paired sequence reads, respectively, each set representing approximately 1% of the genome.
arietinum ICC4958 and C. reticulatum PI489777) were sequenced using the SOLiD (Applied Biosystems) platform to generate 28 Gb (37.8X) and 12 Gb (16.2X) high-quality paired short reads (50 bases), respectively.
We used NGSQCToolkit (v2.3, Platel RK, 2012) with stringent criteria (high-quality paired reads with 90% bases above Q20 level were retained) to remove the low-quality paired-end reads or reads containing adaptors.
After trimming low-quality reads using FASTX Toolkit v0.0.13, the high-quality paired reads were aligned against the reference genome of Sus Scrofa (Sscrofa9.2; http://www.ncbi.nlm.nih.gov/assembly/111518/) using the Burrows–Wheeler Alignment Tool BWAA) with allowing up to four mismatches and other default options.
Whole-genome sequencing of one Gayal as well as one RAN and one JBC (Table S1) was performed on a HiSeq 2000 using genomic DNA, and 97.8 Gb of high quality paired-end reads (100 bp) was generated.
