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In our test, TTAS is able to generate high quality libraries from as little as 20 pg short-fragment DNA.
High quality libraries were sequenced on an Illumina HiSeq 2000.
Electropherograms obtained from amplified cDNA and sequencing libraries indicated that high quality libraries were obtained.
Similarly, to create high quality libraries with reduced parental background, skew primers can also be used.
They were high quality libraries (F = 0.85 and 0.66, respectively) without stop codons in full-length DNAs.
We used the high quality libraries described above to generate paired end sequences for DD, F8, and GB.
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It was a high quality library (F = 1.00) (Table 2).
These results showed MLSDS method gave a high quality library even with three DNA synthesizers.
We report in this article on a convenient method for the construction of a high quality library based on combinatorial DNA synthesis.
The highest quality libraries were those estimated to have the highest proportion of inserts above 1.6 Kb (Table 1).
Of the three tested library preparation protocols, the Nextera XT and Mondrian protocols produced the highest quality libraries across all dilutions.
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